Part:BBa_K1835000:Design

Empty gRNA Scaffold

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 81
  Illegal BsaI.rc site found at 58
  

Design Notes

The BsaI sites were devised with enough space to avoid interference and designed to cut themselves out from the produced backbone.

Source

The part has been in use in our lab originally from the Luciano Marrafini Lab originating from S. Peumoniae.